<最近の招待講演-Resent Invited Presentation>

K Muraki, CFS/ME International 2018: RID - Research Innovation and Discovery Conference (2018, Gold Coast, Australia)

K Muraki, TRP channels in arthritis, Scandinavian Congress of Rheumatology (2018, Helsinki, Finland)

K Muraki, 薬物の作用点としてのカチオンチャネル:その多様性から新たな視点を探る. 2014年度 京都薬科大学 創薬科学フロンテフィアシンポジウム (2014, 京都)

K Muraki, Transcriptional regulation of TRPA1 channel: Links between inflammation and channel expression, Finish Pharmacological Society, Annual meeting 2014 (2014, Tampere, Finland)

 

<最近の発表論文-Resent Publications, 2014~2018>

誌上発表

2018

A Minard, CC Bauer, DJ Wright, HN Rubaiy, K Muraki, DJ Beech & RS Bon. Remarkable progress with small-molecule modulation of TRPC1/4/5 channels: Implications for understanding the channels in health and disease. Cells 7(6), 52 (2018). Review Article

 

T Suzuki#, Y Muraki##, N Hatano##, H Suzuki## & K Muraki#. PIEZO1 channel is a potential regulator of synovial sarcoma cell-viability. Int. J. Mol. Sci. 19 (5), e1452 (2018). #, ##: equal contributions

Keypoint & 臨床との接点:機械刺激受容チャネルPIEZO1がヒト滑膜肉腫の細胞活性を制御する可能性を見出しました!!

2017

K Muraki, K Ohnishi, A Takezawa, H Suzuki, N Hatano, Y Muraki, N Hamzah, R Foster, H Waldmann, P Nussbaumer, M Christmann, RS Bon & DJ Beech. Na+ entry through heteromeric TRPC4/C1 channels mediates (-)Englerin A-induced cytotoxicity in synovial sarcoma cells. Sci. Rep. 7(1), 16988 (2017).

Keypoint & 臨床との接点:(-)-Englerin ATRPC1/4/5チャネルヘテロマを活性化し、ヒト滑膜肉腫に抗がん作用を及ぼすことを見出しました!!

 

K Muraki, T Sekine, Y Ando, H Suzuki, N Hatano, T Hirata & Y Muraki. An environmental pollutant, 9,10-phenanthrenequinone, activates human TRPA1 via critical cysteines 621 and 665. Pharmacol. Res. & Persp. 5, e00342 (2017).

Keypoint & 臨床との接点:環境汚染物質9,10-フェナンスレンキノンの短期曝露がTRPA1チャネルの活性化を介して、気道系組織の生理機能に影響する可能性を見出しました!!

 

Rubaiy HN, Ludlow MJ, Henrot M, Gaunt HJ, Miteva K, Cheung SY, Tanahashi Y, Hamzah N, Musialowski KE, Blythe NM, Appleby HL, Bailey MA, McKeown L, Taylor R, Foster R, Waldmann H, Nussbaumer P, Christmann M, Bon RS, Muraki K* & Beech DJ*. Picomolar, selective and subtype specific small-molecule inhibition of TRPC1/4/5 channels. J. Biol. Chem. 292, 8158-8173 (2017). *: Corresponding Authors

Keypoint & 臨床との接点:TRPC1/4/5チャネルヘテロマを極めて強力に阻害する薬物(Pico145と命名)を初めて見出し、TRPC1/4/5の生理機能を明らかにしました!!

 

K Sakamoto, Y Suzuki, H Yamamura, S Ohya, K Muraki & Y Imaizumi, Molecular mechanisms underlying pimaric acid-induced modulation of voltage-gated K+ channels. J. Pharmacol Sci., 133, 223-231 (2017).

 

MJ Ludlow, HJ Gaunt, HN Rubaiy, KE Musialowski, NM Blythe, NS Vasudev, K Muraki & DJ Beech, (-)-Englerin A-evoked Cytotoxicity is Mediated by Na+ Influx and Counteracted by Na+ /K+-ATPase. J. Biol. Chem. 292, 723-731 (2017).

Keypoint & 臨床との接点:(-)-Englerin Aの抗がん作用には、TRPC1/4の活性化を介したがん細胞へのNa+負荷が重要であることが明らかになりました!!

 

M Fujimoto, T Inoue, H Kito, S Niwa, T Suzuki, K Muraki & S Ohya, Transcriptional repression of HER2 by ANO1 Cl- channel inhibition in human breast cancer cells with resistance to trastuzumab. Biochem. Biophys. Res. Comm. 482, 188-194 (2017).

2016

H Suzuki*, E Sasaki#, A Nakagawa#, Y Muraki, N Hatano & K Murak*. Diclofenac, a non-steroidal anti-inflammatory drug, is an antagonist of human TRPM3 isoforms. Pharmacol. Res. & Persp. 4, e00232 (2016). *, #: equal contributions

Keypoint & 臨床との接点:鎮痛薬として汎用されるジクロフェナク(ボルタレン)がTRPM3チャネルの阻害作用を有することが明らかになりました!!

 

S. Ohya*, H. Kito, N. Hatano & K. Muraki*, Recent advances in therapeutic strategies that focus on the regulation of ion channel expression. Pharmacol & Therap 160, 11-43 (2016). Review Article, *: Corresponding Authors

 

S. Ohya, S. Kanatsuka, N. Hatano, H. Kito, A. Matsui, M. Fujimoto, S. Matsuba, S. Niwa, P. Zhan, T. Suzuki & K. Muraki, Downregulation of the Ca2+-activated K+ channel KCa3.1 by histone deacetylase inhibition in human breast cancer cells. Pharmacol. Res. & Persp. 4, e00228 (2016).

 

Moilanen LJ, Hämäläinen M, Lehtimäki L, Nieminen RM, Muraki K & Moilanen E. Pinosylvin Inhibits TRPA1-Induced Calcium Influx In Vitro and TRPA1-Mediated Acute Paw Inflammation In Vivo. Basic Clin. Pharmacol. Toxicol. 118, 238-242 (2016).

Keypoint & 臨床との接点:松に含まれる生薬成分のピノシルビンがTRPA1チャネルの阻害作用を介して炎症性浮腫を抑制することが明らかになりました!!

 

J Naylor, A Minard, H Gaunt, M Amer, L Wilson, M Migliore, S Cheung, H Rubaiy, N Blythe, K Musialowski, M Ludlow, W Evans, B Green, H Yang, Y You, J Li, C Fishwick, K Muraki, D Beech & R Bon. Natural and synthetic flavonoid modulation of TRPC5 channels. Brit. J. Pharmacol., 173, 562-574 (2016).

Keypoint & 臨床との接点:プロポリスなどに含まれる生薬成分のガランジン(ガランギン)およびその新規誘導物質がTRPC4TRPC5チャネル阻害作用を有することを明らかにしました!!

2015

Y. Akbulut, H. Gaunt, K. Muraki, M. Ludlow, M. Amer, A. Bruns, N. Vasudev, L. Radtke, M. Willot, S. Hahn, T. Seitz, S. Ziegler, M. Christmann, D.  Beech & H. Waldmann.  (-)-Englerin A is a potent and selective activator of TRPC4 and TRPC5 calcium channels. Angewandte Chemie Int. Ed., 54, 3787-3791 (2015). Hot paper

Keypoint & 臨床との接点:アフリカ産植物由来の(-)-Englerin ATRPC4チャネルの活性化を介して選択的に腎がんを抑制することが明らかになりました!!Innovations Report, Spotlight in ACS chemical biology, Chemical & Engineering Newsでも紹介されています)

 

K. Muraki, N. Hatano*, H. Suzuki*, Y Muraki*, Y. Iwajima, Y. Maeda & H. Ono. Oseltamivir blocks human neuronal nicotinic acetylcholine receptor-mediated currents. Basic Clin. Pharmacol. Toxicol., 11687-95 (2015). *: equal contributions

Keypoint & 臨床との接点:抗インフルエンザ薬のタミフルがヒトのニコチン性アセチルコリン受容体チャネルを直接抑制するを明らかにしました!!

2014

Li J, Hou B, Tumova S, Muraki K, Bruns A, et al. Piezo1 integration of vascular architecture with physiological force. Nature, 515, 279-282 (2014).

Keypoint & 臨床との接点:piezo1チャネルが血管内皮の力覚応答を介して血管発生に関わることが明らかになりました!!

 

K. Muraki & H. Ono. Human nicotinic acetylcholine receptor is a potential pharmacological target of oseltamivir. Receptors & Clin. Invest. Research Highlight, 1, e360 (2014).

 

S. Matsuba, S. Niwa, K. Muraki, S. Kanatsuka, Y. Nakazono, N. Hatano, M. Fujii, P. Zhan, T. Suzuki & S. Ohya. Downregulation of Ca2+-activated Cl- channel TMEM16A by the inhibition of histone deacetylase in TMEM16A-expressing cancer cells. J. Pharmacol. Exp. Therap., 351, 510-518 (2014).

 

H. Suzuki, N. Hatano, Y. Muraki, Y. Itoh, S. Kimura, H. Hayashi, K. Onozaki, Y. Ohi, A. Haji & K. Muraki. The NADPH oxidase inhibitor diphenyleneiodonium activates the human TRPA1 nociceptor. Am. J. Physiol., Cell-Physiol. 307, C384-C397 (2014).