古野 忠秀 (ふるの ただひで)

プロフィール

1968年 滋賀県生まれ のどかな田舎町で生まれ育ちました。
1981年 息長小学校卒業 卒業の年は大雪(56豪雪)でした。
1984年 双葉中学校卒業 部活動(野球部)に励んでいました。卒業の年は大雪(59豪雪)でした。
1987年 彦根東高校卒業 ひたすら勉学に勤しみました(?)。いい友達に出会えました。
1991年 名古屋市立大学薬学部卒業 よく遊びました。運よく(?)薬剤師免許を取得できました。運よく面白い研究に出会えました。
1993年 名古屋市立大学大学院薬学研究科博士前期課程修了 薄暗い部屋で顕微鏡を使ってひたすらカルシウムイメージングをしていました。
名古屋市立大学薬学部助手 研究を続ける機会を得ることができました。
1997年 博士(薬学)を授与される 先生方、学生さんにお世話になりました。学位取得後、気持ちを新たに研究に取り組みました。
名古屋市立大学薬学部講師 日本バイオイメージング学会奨励賞を受賞することができました(1999年)。マクマスター大学健康科学部に留学しました(2002-2003年)。日本薬学会奨励賞を受賞することができました(2005年)。
2005年 愛知学院大学薬学部助教授 まっさらの状態から教育に研究に奮闘しました。
2007年 愛知学院大学薬学部准教授 立派な卒業生を送り出し、また、面白い研究をして、世の中の役に立てるよう微力ながら頑張っています。



研究業績

(英語原論文)

1) Nakato, K., Furuno, T., Inagaki, K., Teshima, R., Terao, T., Nakanishi, M.: Cytosolic and intranuclear calcium signals in rat basophilic leukemia cells as revealed by a confocal fluorescence microscope. Eur. J. Biochem., 209, 745-749. (1992)

2) Furuno, T., Isoda, R., Inagaki, K., Iwaki , T., Noji, M., Nakanishi,M.: A fluorescent molecular rotor probes the kinetic process of degranulation of mast cells. Immunol. Lett., 33, 285-288. (1992)

3) Furuno, T., Hamano, T., Nakanishi, M.: Receptor-mediated calcium signal playing a nuclear third messenger in the activation of antigen-specific B cells. Biophys. J., 64, 665-669. (1993)

4) Furuno, T., Hamano, T., Nakanishi, M.: The effects of an inhibitor of the intracellular Ca2+ pump on nuclear calcium signals in B cells. Bioimages, 1, 9-12. (1993)

5) Furuno, T., Okamoto, Y., Hamano, T. Uehara, Y., Nakanishi, M.: Confocal fluorescence microscopy for studying the effects of a tyrosine kinase inhibitor on the nuclear calcium signals in B cells. Bioimages, 1, 175-180. (1993)

6) Teshima, R., Ikebuchi, H., Sawada, J., Furuno, T., Nakanishi, M., Terao, T.: Effects of herbimycin A and ST638 on Fcε receptor-mediated histamine release and Ca2+ signals in rat basophilic leukemia (RBL-2H3) cells. Biochim. Biophys. Acta, 1221, 37-46. (1994)

7) Horikoshi, Y., Furuno, T., Teshima, R., Sawada, J., Nakanishi, M.: Thapsigargin-induced nuclear calcium signals in rat basophilic leukemia cells. Biochem. J., 304, 57-60. (1994)

8) Suzuki-Nishimura, T., Furuno, T., Uchida, K. M., Nakanishi, M.: An initial signal of activation of rat peritoneal mast cells stimulated by Datura stramonium agglutinin: A confocal fluorescence microscopic analysis of intracellular calcium ion and cytoskeletal assembly. Jpn. J. Pharmacol., 66, 205-211. (1994)

9) Kato, E., Akiyoshi, K., Furuno, T., Nakanishi, M., Kikuchi, A., Kataoka, K., Sunamoto, J.: Interaction between ganglioside-containing liposome and rat T-lymphocyte: Confocal fluorescence microscopic study. Biochem. Biophys. Res. Commun., 203, 1750-1755. (1994)

10) Okamoto, Y., Furuno, T., Hamano, T., Nakanishi, M.: Confocal fluorescence microscopy for studying thapsigargin-induced bivalent-cation entry into B cells. Biochem. J., 305, 1011-1015. (1995)

11) Nakanishi, M., Furuno, T., Teshima, R.: Confocal fluorescence microscopy for studying signal transduction in mast cells and basophils. Zoological Studies, 34, 38-40. (1995)

12) Furuno, T., Teshima, R., Kitani, S., Sawada, J., Nakanishi, M.: Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE receptors. Biochem. Biophys. Res. Commun., 219, 740-744. (1996)

13) Okabe, T., Teshima, R., Furuno, T., Torigoe, C., Sawada, J., Nakanishi, M.: Confocal fluorescence microscopy for antibodies against a highly conserved sequence in SH2 domains. Biochem. Biophys. Res. Commun., 223, 245-249. (1996)

14) Sumida, M., Niwata, S., Tanaka, T., Furuno, T., Nakanishi, M., Wakabayashi, K., Boger, P.: Accumulation of protoporphyrinogen IX induced by acifluorfen methyl. Z. Naturforsch., 51c, 174-178. (1996)

15) Takeuchi, K., Ishihara, M., Kawaura, C., Noji, M., Furuno, T., Nakanishi, M.: Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection. FEBS Lett., 397, 207-209. (1996)

16) Ohyama, N., Furuno, T., Nakanishi, M.: Crossbridge rigidity acts differently on mIgM and IgE-mediated calcium signals. Bioimages, 4, 157-159. (1996)

17)  Shimizu , N., Kawazoe, Y., Furuno, T., Nakanishi, M.: Internalization of non-permeant materials into cultured mammalian cells by vortex-stirring in the presence of a high molecular weight polyacrylic acid : Direct evidence of internalization and its dependence on the cell lines. Biol. Pharm. Bull., 20, 727-731. (1997)

18) Nakanishi, M., Noji, M., Furuno, T., Kawaura, C., Nozaki, T.: Intracellular localization of antisense DNA transferred by cationic liposomes with a cationic cholesterol derivative. Cell Vision, 4, 128-129. (1997)

19) Kawaura, C., Furuno, T., Nakanishi, M.: AFM images of cationic liposomes complexed with plasmid DNA. Bioimages, 5, 121-125. (1997)

20) Kawaura, C., Noguchi, A., Furuno, T., Nakanishi, M.: Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative. FEBS Lett., 421, 69-72. (1998)

21) Noguchi, A., Furuno, T., Kawaura, C., Nakanishi, M.: Membrane fusion plays an important role in gene transfection mediated by cationic liposomes. FEBS Lett., 433, 169-173. (1998)

22) Nakamura, R., Furuno, T., Nakanishi, M.: Atomic force microscopy for the cellular localization of secretory granules and coated pits in rat basophilic leukemia cells after antigen stimulation. Bioimages, 6, 69-75. (1998)

23) Suzuki, M., Furuno, T., Teshima, R., Sawada, J., Nakanishi, M.: Soluble factors from rat basophilic leukemia cells stimulated cooperatively the neurite outgrowth of PC12 cells. Biol. Pharm. Bull., 21, 1267-1270. (1998)

24) Parashar, A., Akhand, A. A., Rawar, R., Furuno, T., Nakanishi, M., Kato, M., Suzuki, H., Nakashima, I.: Mercuric chloride induces increases in both cytoplasmic and nuclear free caicium ions through a protein phosphorylation-linked mechanism. Free Radic. Biol. Med., 26, 227-231. (1999)

25) Furuno, T., Ohyama, N., Nakanishi, M.: The relation between degranulation and rapid metabolic responses in RBL-2H3 cells. Biol. Pharm. Bull., 22, 310-312. (1999)

26) Tenjinbaru, K., Furuno, T., Hirashima, N., Nakanishi, M.: Nuclear translocation of green fluorescent protein-nuclear factor κB with a distinct lag time in living cells. FEBS Lett., 444, 1-4. (1999)

27) Suzuki, R., Furuno, T., McKay, D.M., Wolvers, D., Teshima, R., Nakanishi, M., Bienenstock, J.: Direct neurite-mast cell (RBL) communication in vitro occurs via the neuropeptide substance P. J. Immunol., 163, 2410-2415. (1999)

28) Teshima, R., Nakamura, R., Furuno, T., Nakanishi, M.: Degranulation in mast cells and basophils: Confocal and atomic force microscopy. Bioimages, 7, 97-103. (1999)

29) Dace, A., Zhao, L., Park, K.S., Furuno, T., Takamura, N., Nakanishi, M., West, B., Hanover, J.A., Cheng, S.Y.: Hormone binding induces rapid proteasome-mediated degradation of thyroid hormone receptors. Proc. Natl. Acad. Sci. USA., 97, 8985-8990. (2000)

30) Ohshiro, H., Suzuki, R., Furuno, T., Nakanishi, M.: Atomic force microscopy to study direct neurite-mast cell (RBL) communication in vitro. Immunol. Lett., 74, 211-214. (2000)

31) Suzuki, R., Furuno, T., Teshima, R., Nakanishi, M.: Bi-directional flow of in vitro mast cell-nerve communication observed by confocal laser scanning microscopy. Biol. Pharm. Bull., 24, 291-294. (2001)

32) Furuno, T., Hirashima, N., Onizawa, S., Sagiya, N. & Nakanishi, M.: Nuclear shuttling of mitogen-activated protein (MAP) kinase (extracellular signal-regulated kinase (ERK) 2) was dynamically controlled by MAP/ERK kinase after antigen stimulation in RBL-2H3 cells. J. Immunol., 166, 4416-4421. (2001)

33) Amano, T., Furuno, T., Hirashima, N., Ohyama, N. & Nakanishi, M.: Dynamics of intracellular granules with CD63-GFP in rat basophilic leukemia cells. J. Biochem., 129, 739-744. (2001)

34) Furukawa, Y., Furuno, T., Teshima, R., Nakanishi, M.: Calcium signals in rat basophilic leukemia (RBL-2H3) cells primed with the neuropeptide substance P. Biol. Pharm. Bull., 24, 1060-1063. (2001)

35) Ozaki, Y., Blomgren, K., Sugiura-Ogasawara, M., Aoki, K., Furuno, T., Nakanishi, M., Sasaki, M., Suzumori, K.: Role of calpain in human sperm activated by progesterone for fertilization. Biol. Chem., 382, 831-838. (2001)

36) Mizukoshi, T., Kodama, T. S., Fujiwara, Y., Furuno, T., Nakanishi, M., Iwai, S.: Structural study of DNA duplexes containing the (6-4) photoproduct by fluorescence resonance energy transfer. Nucleic Acids Res., 29, 4948-4954. (2001)

37) Noguchi, A., Hirashima, N., Furuno, T., Nakanishi, M.: Activation of receptor tyrosine kinases promotes gene transfection in rat neuronal PC12 cells by cationic liposomes. Neurosci. Lett., 325, 29-32. (2002)

38) Mori, N., Suzuki, R., Furuno, T., McKay, D.M., Wada, M., Teshima, R., Bienenstock, J., Nakanishi, M.: Neurite-mast cell (RBL) communication: RBL membrane ruffling is restricted to the specific site of contact with the activated neurite. Am. J. Physiol. Cell Physiol., 283, C1738-C1744. (2002)

39) Noguchi, S., Hirashima, N., Furuno, T., Nakanishi, M.: Remarkable induction of apoptosis in cancer cells by a novel cationic liposome complexed with a bcl-2 antisense oligonucleotide. J. Contol. Release, 88, 313-320. (2003)

40) Ohyama, N., Furuno, T., Hirashima, N., Nakanishi, M.: The effects of ITIM-bearing FcγRIIB on the nuclear shuttling of MAP kinase in RBL-2H3 cells. Immunol. Lett., 90, 173-176. (2003)

41) Furuno, T., Ma, D., van der Kleij, H. M. P., Nakanishi, M., Bienenstock, J.: Bone marrow-derived mast cells in mice respond in co-culture to scorpion venom activation of superior cervical ganglion neurites according to level of expression of NK-1 receptors. Neurosci. Lett., 372, 185-189. (2004)

42) Suzuki, A., Suzuki, R., Furuno, T., Teshima, R., Nakanishi, M.: N-cadherin plays a role in the synapse-like structures between mast cells and nerves. Biol. Pharm. Bull., 27, 1891-1894. (2004)

43) Furuno, T., Ito, A., Koma, Y., Watabe, K., Yokozaki, H., Bienenstock, J., Nakanishi, M., Kitamura, Y.: The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves. J. Immunol., 174, 6934-6942. (2005)

44) Furuno, T., Nakanishi, M.: Live cell imaging to study signaling molecules in allergic reactions (review). Biol. Pharm. Bull., 28, 1551-1559. (2005)

45) Suzuki, A., Suzuki, R., Furuno, T., Teshima, R., Nakanishi, M.: Calcium response and FcεRI expression in bone marrow-derived mast cells co-cultured with SCG neurites. Biol. Pharm. Bull., 28, 1963-1965. (2005)

46) Furuno, T., Nakanishi, M.:Neurotrophic factors increase tumor necrosis factor-a-induced nuclear translocation of NF-κB in rat PC12 cells. Neurosci. Lett., 392, 240-244. (2006)

47) Kawamura, F., Hirashima, N., Furuno, T., Nakanishi, M.: The effects of 2-methyl-1,4-nephtoquinone (menadione) on cellular signaling in RBL-2H3 cells. Biol. Pharm. Bull., 29, 605-607. (2006)

48) Nakamura, R., Furuno, T., Nakanishi, M.: The plasma membrane shuttling of CAPRI is related to regulation of mast cell activation. Biochem. Biophys. Res. Commun., 347, 363-368. (2006)

49) Amano, K., Furuno, T., Nakanishi, M.: Conditioned medium from feeder STO cells increases the attachment of mouse embryonic stem cells. Biol. Pharm. Bull., 29, 1747-1750. (2006)

50) Ishihara, Y., Furuno, T., Nakanishi, M.: The role of phosphatidylinositol 4-kinase type IIα on degranulation from RBL-2H3 cells. Inflamm. Res., 55, 465-468. (2006)

51) Chikamatsu, S., Furuno, T., Kinoshita, Y., Inoh, Y., Hirashima, N., Teshima, R., Nakanishi, M.: Effects of Cot expression on the nuclear translocation of NF-κB in RBL-2H3 cells. Mol. Immunol., 44, 1490-1497. (2007)

52) Ueno, Y., Hirashima, N., Inoh, Y., Furuno, T., Nakanishi, M.: Characterization of biosurfactant-containing liposomes and their efficiency for gene transfection. Biol. Pharm. Bull., 30, 169-172. (2007)

53) Ueno, Y., Inoh, Y., Furuno, T., Hirashima, N., Kitamoto, D., Nakanishi, M.: NBD-conjugated biosurfactant (MEL-A) shows a new pathway for transfection. J. Control. Release, 123, 247-253. (2007)

54) Suzuki, R., Furuno, T., Okamoto, K., Teshima, R., Nakanishi, M.: ATP plays a role in neurite stimulation with activated mast cells. J. Neuroimmunol., 192, 49-56. (2007)

55) Obata, K., Furuno, T., Nakanishi, M., Togari, A.: Direct neurite-osteoblastic cell communication, as demonstrated by use of an in vitro co-culture system. FEBS Lett., 581, 5917-5922. (2007)

56) Kumagai, K., Ozaki, Y., Nakanishi, T., Inomata, M., Furuno, T., Nakanishi, M., Ogasawara, M. S.: Role of μ-calpain in human decidua for recurrent miscarriage. Am. J. Reprod. Immunol., 59, 339-346. (2008)

57) Koma, Y., Furuno, T., Hagiyama, M., Hamaguchi, K., Nakanishi, M., Masuda, M., Hirota, S., Yokozaki, H., Ito, A.: Cell adhesion molecule 1 is a novel pancreatic-islet cell adhesion molecule that mediates nerve-islet cell interactions. Gastroenterology, 134, 1544-1554. (2008)

58) Nakanishi, M., Furuno, T.: Molecular basis of neuroimmune interaction in an in vitro coculture approach (review). Cell. Mol. Immunol., 5, 249-259. (2008)

59) Sugimoto, Y., Furuno, T., Nakanishi, M.: Effect of NeuroD2 expression on neuronal differentiation in mouse embryonic stem cells. Cell Biol. Int., 33, 174-179. (2009)

60) Inoh, Y., Furuno, T., Hirashima, N., Nakanishi, M.: Non-viral vectors with a biosurfactant MEL-A promote gene transfection into solid tumors in mouse abdominal cavity. Biol. Pharm. Bull., 32, 126-128. (2009)

61) Nakanishi, M., Inoh, Y., Kitamoto, D., Furuno, T.: Nano vectors with a biosurfactant for gene transfection and drug delivery (review). J. Drug Deliv. Sci. Tech., 19, 165-169. (2009)

62) Miyazu, S., Furuno, T., Nakanishi, M.: Phosphorylation states of STAT3 and ERKs in mouse embryonic stem cells. Cell Biol. Int., 34, 485-492. (2010).

63) Hoshino, Y., Hirashima, N., Nakanishi, M., Furuno, T.: Inhibition of degranulation and cytokine production in bone marrow-derived mast cells by hydrolyzed rice bran. Inflamm. Res., 59, 615-625. (2010)

64) Inoh, Y., Furuno, T., Hirashima, N., Kitamoto, D., Nakanishi, M.: The ratio of unsaturated fatty acids in biosurfactants affects the efficiency of gene transfection. Int. J. Pharm., 398, 225-230. (2010)

65) Hagiyama, M., Furuno, T., Hosokawa, Y., Iino, T., Ito, T., Inoue, T., Nakanishi, M., Murakami, Y., Ito, A.: Enhanced nerve-mast cell interaction by a neuronal short isoform of cell adhesion molecule-1, CADM1. J. Immunol., 186, 5983-5992. (2011)

66) Furuno, T., Nakanishi, M.: A rational approach to inducing neuronal differentiation in embryonic stem cells, in Lovrecic, K. (Ed), Embryonic Stem Cells: The Hormonal Regulation of Pluripotency and Embryogenesis, InTech, pp429-444. (2011)

67) Furuno, T., Nakanishi, M.: Analysis of neuro-immune interactions by an in vitro co-culture approach, in Merighi, A. (Ed), Neuropeptides: Methods and Protocols, Methods in Molecular Biology vol. 789, Springer, pp171-180. (2011)

68) Inoh, Y., Furuno, T., Hirashima, N., Kitamoto, D., Nakanishi, M.: Rapid delivery of small interfering RNA by biosurfactant MEL-A-containing liposomes. Biochem. Biophys. Res. Commun., 414, 635-640. (2011).

69) Furuno, T., Nakanishi, M.: Kefiran suppresses antigen-induced mast cell activation. Biol. Pharm. Bull., 35, 178-183. (2012)

70) Takekawa, M., Furuno, T., Hirashima, N., Nakanishi, M.: Mitochondria take up Ca2+ in two steps dependently on store-operated Ca2+ entry in mast cells. Biol. Pharm. Bull., 35, 1354-1360. (2012)

71) Furuno, T., Hagiyama, M., Sekimura, M., Okamoto, K., Suzuki, R., Ito, A., Hirashima, N., Nakanishi, M.: Cell adhesion molecule 1 (CADM1) on mast cells promotes interaction with dorsal root ganglion neurites by heterophilic binding to nectin-3. J. Neuroimmunol., 250, 50-58. (2012)

72) Hagiyama, M., Inoue, T., Furuno, T., Iino, T., Itami, S., Nakanishi, M., Asada, H., Hosokawa, Y., Ito, A.: Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve-mast cell interaction in a hapten-induced mouse model of atopic dermatitis. Br. J. Dermatol., 168, 771-778. (2013)

73) Inoh, Y., Furuno, T., Hirashima, N., Kitamoto, D., Nakanishi, M.: Synergistic effect of a biosurfactant and protamine on gene transfection efficiency. Eur. J. Pharm. Sci., 49, 1-9. (2013)


(日本語)

1) 中西 守、古野忠秀:細胞膜から核へのシグナル.細胞工学, 12, 595-598. (1993)

2) 古野忠秀、中西 守:リンパ球のCa2+動態.新しい光学顕微鏡(第2巻) pp146-148. (1995)

3) 中西 守、古野忠秀、長尾陽子:単一細胞における細胞内カルシウム測定法(免疫細胞).細胞内カルシウム研究プロトコール(実験医学別冊) pp152-158. (1996) 

4) 古野忠秀:トレース分子、ケージド化合物.見る技術(蛋白質 核酸 酵素増刊号) pp1122-1127. (1997)

5) 中村亮介、古野忠秀、中西 守:免疫系における細胞間相互作用.日本物理学会誌, 53, 2-6. (1998)

6) 古野忠秀、中西 守:リンパ球におけるカルシウムイメージング.細胞, 30, 11-14. (1998)

7) 古野忠秀:ストレス応答とカルシウム動態.放射線科学, 41, 49-53. (1998)

8) 中西 守、古野忠秀:花粉症とアレルギー反応 −分子レベルでの解明−.薬局, 49, 455-460. (1998)

9) 古野忠秀、中西 守:細胞のアポトーシスのイメージング.バイオイメージングの最先端, pp161-165. (1999)

10) 古野忠秀、中西 守:細胞内シグナル分子の可視化解析.生物物理, 43, 218-222. (2003)

11) 古野忠秀:顕微光学法によるマスト細胞活性化の分子機構の研究.薬学雑誌, 125, 671-683. (2005)

12) 中西 守、古野忠秀:免疫反応の利用.スタンダード薬学シリーズ4 生物系薬学III 生体防御, pp132-143. (2006)

13) 古野忠秀、中西 守:細胞工学的手法.生物物理学ハンドブック, pp632-634. (2007)

14) 中西 守、古野忠秀:分子イメージング法による細胞内情報伝達機構の研究.薬品分析科学の最前線, pp172-173. (2009)

15) 中西 守、古野忠秀:肥満細胞と感覚神経の接着におけるCADM1とネクチン-3の関与.生体の科学,64, 216-219. (2013)


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